Shigella dysenteriae type 1-specific bacteriophage from environmental waters in Bangladesh

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dc.contributor.authorFaruque, Shah M.-
dc.contributor.authorChowdhury, Nityananda-
dc.contributor.authorKhan, Rase-
dc.contributor.authorHasan, M. Rubayet-
dc.contributor.authorNahar, Jebun-
dc.contributor.authorIslam, M. Johirul-
dc.contributor.authorYamasaki, Shinji-
dc.contributor.authorGhosh, A.N.-
dc.contributor.authorNair, G. Balakrish-
dc.contributor.authorSack, David A.-
dc.date.accessioned2015-05-31T10:21:50Z-
dc.date.available2015-05-31T10:21:50Z-
dc.date.issued2003-
dc.identifier.citationAppl Environ Microbiol 2003 Dec;69(12):7028-31en
dc.identifier.urihttp://hdl.handle.net/123456789/5805-
dc.description.abstractAbstract Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface water samples from Bangladesh that specifically lyses strains of S. dysenteriae type 1. This phage, designated SF-9, belongs to the Podoviridae family and has a 41-kb double-stranded DNA genome. Further screening of water samples for the prevalence of the phage revealed 9 of 71 (12.6%) water samples which were positive for the phage. These water samples were also positive in PCR assays for one or more S. dysenteriae type 1-specific genes, including ipaBCD and stx1, and live S. dysenteriae type 1 was isolated from three phage-positive samples. The results of this study suggest that phage SF-9 may have epidemiological applications in tracing the presence of S. dysenteriae type 1 in environmental watersen
dc.format.extent162904 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectDysentery, bacillary-virologyen
dc.subjectFresh wateren
dc.subjectPodoviridaeen
dc.subjectPolymerase chain reactionen
dc.subjectRestriction mappingen
dc.subjectShigella dysenteriaeen
dc.subjectBacterial proteinsen
dc.subjectBangladeshen
dc.subjectDeoxyribonucleases, Type II Site-Specificen
dc.titleShigella dysenteriae type 1-specific bacteriophage from environmental waters in Bangladeshen
dc.typeArticleen
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