Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein

Full metadata record
DC FieldValueLanguage
dc.contributor.authorNakano, Masayuki-
dc.contributor.authorYamamoto, Shingo-
dc.contributor.authorTerai, Akito-
dc.contributor.authorOgawa, Osamu-
dc.contributor.authorMakino, Sou-Iichi-
dc.contributor.authorHayashi, Hideo-
dc.contributor.authorNair, G.Balakrish-
dc.contributor.authorKurazono, Hisao-
dc.identifier.citationFEMS Microbiol Lett 2001 Nov 27 ; 205(1) : 71-6en
dc.description.abstractA total of 321 uropathogenic Escherichia coli (UPEC) strains and 12 strains of E. coli isolated from stool samples of healthy individuals, which were previously shown to be positive in colony hybridization test using the usp (encoding for the uropathogenic-specific protein) DNA probe, were examined by PCR amplification to determine the size of the usp gene and the pathogenicity island (PI). Three types of size variation were observed for the usp gene and four types for the PI. Sequencing analysis of the PIs from seven representative strains (six UPEC and one from a normal healthy individual) revealed that the usp genes can be classified into two groups, each having different sequences in the 3'-terminal region. The peptides encoded by the three open reading frames (ORFs) downstream of usp had identical 23 amino acid residues in the C-terminal region. The subregion encoding these small ORFs has a mosaic structure constituted of six segments. The positions of these segments vary from strain to strain, and in some strains, two to four segments are deleted. This indicates that rearrangements occur frequently in this region and the mosaic arrangement apparently contributes to the size variation observed in the PCR examination of the usp genes and PIsen
dc.format.extent298309 bytes-
dc.subjectEscherichia coli infectionsen
dc.subjectEscherichia coli proteinsen
dc.subjectUrinary tract infectionsen
dc.subjectSequence analysis, DNAen
dc.subjectCloning, molecularen
dc.subjectAmino acid sequenceen
dc.titleStructural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP proteinen
Appears in Collections:A. Original papers

Files in This Item:
File Description SizeFormat 
2001-FEMSMicrobiolLett-205-NakanoM.pdf291.32 kBAdobe PDFView/Open    Request a copy

This item is protected by original copyright