A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica

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dc.contributor.authorGonzalez-Ruiz, A.-
dc.contributor.authorHaque, R.-
dc.contributor.authorRehman, T.-
dc.contributor.authorAguirre, A.-
dc.contributor.authorJaramillo, C.-
dc.contributor.authorCastañon, G.-
dc.contributor.authorHall, A.-
dc.contributor.authorGuhl, F.-
dc.contributor.authorRuiz-Palacios, G.-
dc.contributor.authorWarhurst, D.C.-
dc.identifier.citationJ Clin Microbiol 1992 Nov;30(11):2807-13en
dc.description.abstractApproximately 10% of the world population is infected with Entamoeba histolytica, but only 10% of the carriers develop symptomatic amebiasis. This discrepancy could be explained by the genotypic differences between the morphologically indistinguishable invasive and noninvasive strains of E. histolytica currently identified by zymodeme analysis, a technique that is unsuitable for routine diagnostic laboratories. Here we report the production of a monoclonal antibody against E. histolytica and its use in an immunofluorescence assay to identify invasive isolates cultured from stool samples of infected patients in several regions where amebiasis is endemic: Bangladesh, Colombia, and Mexico. After testing a total of 88 E. histolytica isolates, the correlation between zymodeme characterization and the immunofluorescence assay with the invasive isolate-specific monoclonal antibody was 100%. The epitope detected by the invasive isolate-specific monoclonal antibody resides in a previously undescribed internal protein with molecular masses of 84 and 81 kDa in axenic and polyxenic E. histolytica strains, respectivelyen
dc.format.extent1873064 bytes-
dc.subjectAntibodies, Monoclonalen
dc.subjectAntigens, Protozoanen
dc.subjectCarrier Stateen
dc.subjectEntamoeba histolyticaen
dc.subjectEvaluation Studies as Topicen
dc.titleA monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolyticaen
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