Contact-haemolysin production by entero-invasive Escherichia coli and shigellae

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dc.contributor.authorHaider, Khaleda-
dc.contributor.authorAlbert, M.J.-
dc.contributor.authorHossain, A.-
dc.contributor.authorNahar, S.-
dc.identifier.citationJ Med Microbiol 1991 Dec;35(6):330-7en
dc.description.abstractEntero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy broth, and for EIEC it was casamino acid-yeast extract broth with 1 mM CaCl2. CH production by all species was best at the slightly alkaline pH which is optimal for growth; it was also dependent on the presence of a large (140-Mda) plasmid. Pre-treatment of bacteria with homologous antisera inhibited CH activity. Various treatments of bacterial cells and RBCs suggested that CH may be a protein molecule, and that a chitotriose-like moiety may serve as CH receptor. RBCs that were incubated with bacteria at 4 degrees C, or with heat-killed bacteria at 37 degrees C, were not lysed; also, isolated cell-surface components (lipopolysaccharide and outer-membrane protein) did not lyse RBCs. This suggests that metabolically active cells are required for CH activity. Production of CH by both EIEC and shigellae is consistent with a common mechanism for the virulence of these organismsen
dc.format.extent1155208 bytes-
dc.subjectEscherichia coli Infectionsen
dc.subjectDysentery, Bacillaryen
dc.subjectTime Factorsen
dc.titleContact-haemolysin production by entero-invasive Escherichia coli and shigellaeen
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