Factors affecting production of haemolysin by strains of vibrio fluvialis
The in vitro production of haemolysin by Vibrio fluvialis was studied using sheep erythrocyte. The effect of the composition of various media and different concentrations of sodium chloride on the production of haemolysin and heat-stability was investigated. Comparatively higher titre of haemolysin production was noted in brain heart infusion (BHI) broth. Adding 0.5% NaCI to BHI broth reduced the production of haemolysin; adding 5.0% NaCI to the medium totally inhibited it. The highest titre of haemolysin was produced at 30 Â°C and 37 Â°C, whereas no haemolysin was produced at 50 Â°C. Haemolytic activity was totally destroyed when heated at 56 Â°C for 30 minutes. Haemolysin could be assayed easily following this method.