Evaluation of a probe hybridisation serotyping method for group A rotavirus

Abstract

Serotype-specificity and sensitivity of oligonucleotide probes to serotype human rotavinises was assessed. Probes could detect as little as 63 ng of homologous RNA and none reacted with as much as 100 ng of hcterologous RNA. Northern-blot analysis revealed that probes reacted with one of genomic segments 7, 8 or 9 of corresponding serotypes