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Detection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCR

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dc.contributor.authorRahman, Mahbubur-
dc.contributor.authorMauff, Gotteried-
dc.contributor.authorLevy, Jack-
dc.contributor.authorCouturier, Martine-
dc.contributor.authorPulverer, Gerhard-
dc.contributor.authorGlasdorff, N.-
dc.contributor.authorButzler, J.P.-
dc.date.accessioned2008-09-17T02:15:25Z-
dc.date.available2008-09-17T02:15:25Z-
dc.date.issued1994-10-
dc.identifier.citationAntimicrob Agents Chemother 1994 Oct;38(10):2488-91en
dc.identifier.urihttp://hdl.handle.net/123456789/1800-
dc.description.abstractTo study 4-quinolone resistance, the N-terminal coding region of gyrA from nalidixic acid-susceptible and -resistant isolates of Shigella dysenteriae type 1 was amplified by PCR, cloned, and sequenced. DNA sequence analysis of gyrA from nalidixic acid-resistant isolates revealed a C-to-T transition at nucleotide position 248 leading to a Ser-83-to-Leu substitution which was absent in susceptible clinical isolates. Direct HinfI digestion of PCR-amplified DNA detected similar mutations. Thus, DNA gyrase A subunit mutation Ser-83 to Leu is implicated in 4-quinolone resistance in S. dysenteriae type 1en
dc.format.extent178733 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectShigella dysenteriaeen
dc.subjectGenes, Bacterialen
dc.subjectQuinolonesen
dc.subjectDysentery, Bacillaryen
dc.subjectPolymerase chain reactionen
dc.subjectShigella dysenteriae-isolation & purificationen
dc.titleDetection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCRen
dc.typeArticleen
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