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Title: Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques.
Authors: Islam, M.S.
Hossian, M.Z.
Khan, S.I.
Felsenstein, A.
Sack, R.B.
Albert, M.J.
Keywords: Shigella dysenteriae
Issue Date: 1997
Citation: J Diarrhoeal Dis Res 1997 Jun;15(2):65-70
Abstract: Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10(5) cfu of S. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that S. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable S. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae.
URI: http://hdl.handle.net/123456789/97
Appears in Collections:Public health sciences research papers

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