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| Title: | Detection of non-culturable Shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and PCR techniques. |
| Authors: | Islam, M.S.
Hossian, M.Z.
Khan, S.I.
Felsenstein, A.
Sack, R.B.
Albert, M.J. |
| Keywords: | Shigella dysenteriae
Epidemiology |
| Issue Date: | 29-Aug-2007 |
| Citation: | J Diarrhoeal Dis Res 1997 Jun;15(2):65-70 |
| Abstract: | Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10(5) cfu of S. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that S. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable S. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae. |
| URI: | http://hdl.handle.net/123456789/97 |
| Appears in Collections: | Public health sciences research papers
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| 1997-JDiarDisRes-65-Islam.pdf | | 348Kb | Adobe PDF | View/Open |
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