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Title: Analysis of human rotavirus G serotype in Bangladesh by enzyme-linked immunosorbent assay and polymerase chain reaction
Authors: Ahmed, M.U.
Alam, M.M.
Chowdhury, N.S.
Haque, M.M.
Shahid, N.
Kobayashi, N.
Taniguchi, K.
Urasawa, T.
Urasawa, S.
Keywords: Rotavirus
Bangladesh
Diarrhea
Infantile
Issue Date: 1999
Citation: J Diarrhoeal Dis Res 1999 Mar;17(1):22-27
Abstract: Distribution of human rotavirus G serotype was investigated by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) with faecal specimens obtained from children with diarrhoea in Bangladesh. By ELISA, subgroup and G serotype were determined for 59.5% and 28.6% of group A rotavirus-positive specimens respectively. However, of the 120 specimens, the G serotype of which was not determined by ELISA, serotype of the 112 specimens was typed by PCR. In total, G serotype was assigned for 95.2% of all the specimens, showing the highest rate of G4 (41.7%), followed by Gl (23.2%) and G2 (14.9%). Twenty-four specimens showed mixed types, such as G2 with Gl, G8 or G9, or Gl with G4. These results indicate that PCR combined with ELISA is highly effective for G serotyping of rotavirus
URI: http://hdl.handle.net/123456789/394
ISSN: 0253-8768
Appears in Collections:Laboratory sciences research papers

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