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Title: Immunoblot analysis as a diagnostic tool for detection of visceral leishmaniasis in Bangladesh
Authors: Iftekhar, Nahid Tofail
Qadri, F.
Rahman, M.
Amin, M.R.
Rahman, K.M.
Keywords: Visceral Leishmaniasis
Issue Date: 20-Nov-2007
Series/Report no.: J Diarrhoeal Dis Res
1998 Mar;16(2):97
Abstract: Objective: Develop a specific diagnostic test for visceral leishmaniasis in Bangladesh. Methodology: Sera of 32 confirmed visceral leishmaniasis (VL) patients, obtained from different hospitals in Bangladesh during November 1996-April 1997, were studied by the immunoblot technique with antigen prepared from Leishmania donovani. Controls included sera of 34 healthy individuals from both endemic and non-endemic regions, 25 patients with non-lcishmanial infections, and one individual treated for visceral leishmaniasis. Direct agglutination test (DAT) was performed on all sera. Results: Sera of the VL patients showed heterogeneity of polypeptide recognition and identified many polypeptides with relative molecular mass ranging from 16 to > 106 kD. The 56-64-kD band was recognized by all sera, while the 106, 78, 76 and 66-kD polypeptide bands were identified by 91%, 91%, 97%, and 97% of the sera from the VL patients respectively. Three of these polypeptides and the 56-64 kD polypeptide were recognized by 97% of the sera from the VL patients. The 76-kD polypeptide band was not recognized by sera of only two patients, of whom one had been treated for VL. The recognition of the 56-64-kD band had a sensitivity and specificity of 100% and 90% respectively and that of the 76-kD band has a sensitivity and specificity of 97% and 98%. For both VL patients * and the treated individual, DAT was positive at high litre (1:102400). The sera of patients with non-leishmanial infection identified one or two of the five specific polypeptides, but in no case more than two. Conclusion: Immunoblot analysis can be a valuable tool in specific diagnosis of active visceral leishmaniasis in ^ ^ Bangladesh. The recognition of the 56-64-kD band, in addition to any three bands, may be considered diagnostic of - * VL. Additionally, further studies can confirm if this technique can differentiate active infection from treated infections unlike DAT, which is currently used in Bangladesh.
URI: http://hdl.handle.net/123456789/356
ISSN: 0253-8768
Appears in Collections:Infectious diseases and vaccine sciences conference papers

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