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Title: Evaluation of a non-radioactive chemiluminescent method for using oligonucleotide and polynucleotide probes to identify enterotoxigenic Escherichia coli
Authors: Alim, A.R.M. Abdul
Faruque, Shah M.
Ahmad, Q.S.
Hossain, K.M. Belayet
Mahalanabis, Dilip
Albert, M. John
Keywords: Enterotoxins-biosynthesis
Escherichia coli-isolation & purification
Molecular Sequence Data
Nucleic Acid Hybridization
Child, Preschool
DNA Probes
DNA, Bacterial-isolation & purification
Issue Date: Jun-1994
Citation: J Diarrhoeal Dis Res. 1994 Jun;12(2):113-6
Abstract: We compared the applicability of an enhanced chemiluminescent (ECL) method for using gene probes with that of radioactive probes to identify enterotoxigenic Escherichia coli (ETEC) in stools of Bangladeshi children with diarrhoea. Colony blots of E. coli isolates were hybridized using both [alpha-32P]-dCTP labelled and fluorescein-11-dUTP labelled polynucleotide and oligonucleotide gene probes for heat-labile enterotoxin (LT), and heat-stable enterotoxin (ST). Analysis of 1,620 isolates obtained from 540 patients gave similar results by both radioactive and chemiluminescent probes. The ECL method was faster than the radioactive method. Both polynucleotide and oligonucleotide probes could be used by the ECL method. Hybridization and detection by the ECL method appeared to be a convenient alternative to radioactive probes for screening E. coli isolates for ETEC
URI: http://hdl.handle.net/123456789/3048
Appears in Collections:Laboratory sciences research papers

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