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| Title: | Association of plasmids with the carbohydrate composition of the o-antigen in shigella dysenteriae type 1 |
| Authors: | Hossain, M.L.
Haider, Khaleda
Chowdhury, T.A.
Mosihuzzaman, M. |
| Keywords: | Cholera
O Antigen
Shigella dysenteriae |
| Issue Date: | 1-Nov-2007 |
| Series/Report no.: | J Diarrhoeal Dis Res
1993 Jun;11(2):93-6 |
| Abstract: | Six different prepared mutant strains of Shigella dysenteriae type 1 with various combinations of 'core plasmids of 140, 6, and 2 Mdal, were cultivated separately and their hpoporvsaccharide (LPS) samples were isolated, which on delipidation afforded polysaccharidcs and oligosaccharides. The LPS and prepared O-antigen porysaccharides were subjected to acid hydrolysis, and the constituent sugars were analysed by paper chromatography and gas liquid chromatography (GLC), using CP Sil 8 CB fused silica capillary column equipped with a flame ionizatioa detector. The mutant strains, harbouring the 6 Mdal plasmid, contained rhamnose, galactosc, and N-acctyi glucosamine in their O-antigen polysaccharides with a minor variation in the relative proportions of these sugar constituents as compared to those of the control strain. The sugar composition of the O-antigen porysaccharides prepared from the mutant strains, without the 6 Mdal plasmid but containing 2 or 140 Mdal plasmid, contained galactose and N-acetyl glucosamine but no rhamnose. However, mutant strains with none of the core plasmids contained traces of rhamnose and were devoid of N-acctyl ghicosaminc, but contained heptose. These results suggest that 6 Mdal plasmid is essentially required for the complete synthesis of O-antigen polysaccharidcs. However, the 140 and 2 Mdal plasmids might also have an association with synthesis of carbohydrate composition of the O-antigen in S. dysenteriae type 1. |
| URI: | http://hdl.handle.net/123456789/271 |
| ISSN: | 0253-8768 |
| Appears in Collections: | Public health sciences research papers
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| 1993-JDiarDisRes-93-HossainML.pdf | | 177Kb | Adobe PDF | View/Open |
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