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| Title: | Survival of Shigella dysenteriae Type 1 on Fomites |
| Authors: | Islam, Md. Sirajul
Hossain, M.A
Khan, S.I.
Khan, M.N.H.
Sack, R.B.
Albert, M.John
Huq, A.
Colwell, R.R. |
| Keywords: | Cholera
Shigella dysenteriae |
| Issue Date: | 2-Sep-2007 |
| Series/Report no.: | J Health Popul Nutr
2001 Sep;19(3):177-182 |
| Abstract: | Studies have shown that various objects, such as utensils, toys,a nd clothes, can serve as vehicles for transmission of Shigella spp. Shigellae can become viable but non-culturable (VBNC)when exposed to various environmental conditions as shown in earlier studies. The present study was carried out to detect VBNC Shigella dysenteriae type 1 on various fomites by direct viable counting, polymerase chain reaction (PCR), and fluorescent antibody methods.S.dysenteriae type 1 was inoculated onto cloth, wood, plastic, aluminum, and glass objects. Results showed that 1.5-4.0 hours after inoculation, S.dysenteriae type 1 became non-culturable, and after five days,non-culturable but viable S. dysenteriae type 1 could be detected by both PCR and fluorescent antibody techniques. Fomites can be considered an important potential route of transmission of VBNC S.dysenteriae type 1 and a significant factor in the epidemiology of shigellosis. |
| URI: | http://hdl.handle.net/123456789/114 |
| Appears in Collections: | Public health sciences research papers
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Size | Format |
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| 2001-JHealthPopuNutr-177-Islam.pdf | | 191Kb | Adobe PDF | View/Open |
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